Avian and Reptilian Developmental Biology by Guojun Sheng

Author:Guojun Sheng
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

2.Insert a glass capillary from the tip of the forebrain of the HH stages 10–12 embryos. When the tip of the glass capillary reached inside of the optic vesicle, inject a solution of a mixture of pCAGGS-T2TP and pT2K-CAGGS construct into the optic vesicle. Be careful not to leak the DNA solution outside of the optic vesicle.

3.Place the electrodes (2 mm distance) on the vitelline membrane to be rectangular to the nasal-temporal axis of the optic vesicle (Fig. 2a, b). If you target the nasal half of the eye, place the cathode just anterior of the optic vesicle and anode rectangular to the optic vesicle. To label the temporal half of the eye, the anode places just posterior to the caudal side of the optic vesicle, and cathode places anterior to the rostral side.

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